human acute t lymphocyte lines jurkat  (ATCC)

Journal: Cells

Article Title: Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs)

doi: 10.3390/cells12182294

Figure Lengend Snippet: PEP-1 decreases the mRNA expression level of IL-2 and IL-2Rα in Jurkat cells. The level of IL-2 ( A ) and IL-2Rα ( B ) mRNA relative expression was evaluated by qPCR in Jurkat cells treated or not with GILZ peptides (0.1 μM) for 1 h. Afterward, cells were activated with PMA and Ionomycin for 6 h and treated with acetonitrile alone (vehicle), scramble, or PEP peptides (0.1 μM), as indicated in the figure. Graphs represent the mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.005, **** p < 0.0001, P -values were calculated according to unpaired Student’s t -test. ns: non-significant.

Article Snippet: Jurkat human T lymphocytic leukemia cell line was purchased from the American Type Culture Collection, (ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Cells

Article Title: Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs)

doi: 10.3390/cells12182294

Figure Lengend Snippet: PEP-1 decreases pNF-κB/p65 activation in Jurkat cells. Representative dot plots of flow cytometry analysis of p-NF-κB/p65 in Jurkat cells stimulated or not with PMA and ionomycin for 15 min ( A ). Numbers within quadrants represent the frequency of the gated population of positive pNF-κB cells treated with scramble or PEP-1 peptide (5 μM), compared to the frequency of positive pNF-κB unstimulated Jurkat cells. After that, graphs represent the frequency of pNF-κB+ live Jurkat cells treated with different doses of PEP-1 at the concentration indicated in the above graphs ( B ). Graphs represent the mean of at least three independent experiments ± SD. * p < 0.05. p -values were calculated using the unpaired Student’s t -test. ns: non-significant.

Article Snippet: Jurkat human T lymphocytic leukemia cell line was purchased from the American Type Culture Collection, (ATCC, Manassas, VA, USA).

Techniques: Activation Assay, Flow Cytometry, Concentration Assay

Journal: Cancer Communications

Article Title: Enhancement of CAR‐T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins

doi: 10.1002/cac2.12452

Figure Lengend Snippet: EGFR‐CAR T cells and B7H3‐CAR T cells exhibited general antitumor activity in cholangiocarcinoma cell lines. (A‐B) Detection of tumor tissue antigens collected from patients with cholangiocarcinoma by IHC staining. The left and right panels of A displayed representative EGFR and B7H3 expression in normal, para‐carcinoma area, and tumor zone with enlarged view (5x, scale bar = 200 μm; 20x, scale bar = 50 μm). Statistical analysis of EGFR and B7H3 antigens scores in normal, para‐carcinoma and carcinoma tissues (B, n = 20). (C) Schematic representation of the lentiviral vectors carrying an EGFR‐specific 3 rd generation CAR moiety (EGFR‐CAR) and a B7H3‐specific 3 rd generation CAR moiety (B7H3‐CAR). (D) Immunoblotting analysis of EGFR‐CAR and B7H3‐CAR expression were performed with anti‐flag tag. The positive control was GP120‐CAR. (E‐F) Detection cytotoxic activities of the EGFR‐CAR‐T cells and the B7H3‐CAR‐T cells with TFK‐1 cells and HuCCT1 cells by LDH assay. The negative control was Jurkat cells without EGFR and B7H3 expression ( n = 3, healthy donors). Experiments were performed independently at least 3 times. One‐way ANOVA was used in Tukey's multiple comparison test. Experimental data are expressed as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Abbreviations: CAR, chimeric antigen receptor T; EGFR, epidermal growth factor receptor; B7H3, B7 homolog 3; GP120, gp120 antigen; IHC, immunohistochemistry; LDH, lactate dehydrogenase; SEM, standard error of the mean.

Article Snippet: Human embryonic kidney cell line HEK293T, human cholangiocarcinoma cell line TFK‐1 and HuCCT1, and human acute T‐lymphocytic leukemia cell line Jurkat were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, Immunohistochemistry, Expressing, Western Blot, FLAG-tag, Positive Control, Lactate Dehydrogenase Assay, Negative Control, Comparison

Journal: Cell Death & Disease

Article Title: Tumor exosome promotes Th17 cell differentiation by transmitting the lncRNA CRNDE-h in colorectal cancer

doi: 10.1038/s41419-020-03376-y

Figure Lengend Snippet: A Naive CD4 + T cells treated with CRC cell-exosome (SW480-E, HT29-E, and LOVO-E) for 72 h, and then the protein level of RORγt was detected by western blot. B Naive CD4 + T cells treated with exosome derived by CRC cells (SW480, HT29, and LOVO) were transfected with si-CRNDE-h (or si-control) for 72 h. Later, the protein level of RORγt was detected by western blot. C Jurkat cells were transfected with IL-17 promoter-driven luciferase vector, and then treated with CRC cell-exosome (SW480-E, HT29-E, and LOVO-E) for 72 h. Later, luciferase activity was detected. D Jurkat cells were transfected with IL-17 promoter-driven luciferase vector, and then treated with exosome derived by CRC cells (SW480, HT29, and LOVO) transfected with si-CRNDE-h (or si-control) for 72 h. Later, luciferase activity was detected. E: exosome. * p < 0.05, ** p < 0.01 vs. NCM460-E or si-control-E.

Article Snippet: The human acute T-lymphocytic leukemia cell line (Jurkat) was purchased from ATCC (USA) and was cultured in RPMI-1640 supplemented with 10% FBS.

Techniques: Western Blot, Derivative Assay, Transfection, Control, Luciferase, Plasmid Preparation, Activity Assay